Here we report on a technical difficulty we encountered while optimizing genotyping strategies to identify mice derived from Exoc3l2tm1a(KOMP)Wtsi embryonic stem cells obtained from the Knockout Mouse Project Repository. The Exoc3l2tm1a(KOMP)Wtsi construct encodes a "knockout-first" design with loxP sites that confer conditional potential (KO1st). We designed primers that targeted wil... https://www.roneverhart.com/Gretsch-G5210T-P90-Electromatic-Jet-Two-90-Single-Cut-Electric-Guitar-w-Bigsby-Mako-p23063/